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投稿时间：2014-09-09
投稿时间：2014-09-09
中文摘要: 大麦不同溶剂提取物中多糖结构及其降血糖效果差异的研究。 用水、30 %乙醇、70 %乙醇对大麦进行提取，最 终得水提醇沉物、30 %乙醇提醇沉物、70 %乙醇提取物。将各提取物分别灌胃给予链脲佐菌素诱导的糖尿病小鼠，检测 其降血糖作用。用 DEAE-cellulose 柱、HW-55F 柱和 Sephacryl S-400 柱分别对有降血糖作用的水提醇沉物、30 %乙醇提 醇沉物进行分离提纯，各得到一种多糖。多糖（BP）I 为呋喃葡萄糖分子以 1→3 键相连，α 和 β 构型交替出现，无侧链，分 子式为（C21H33O16）n；多糖（BP）II 为半乳糖分子以 1→4 键相连形成主链，另有一个半乳糖分子连在主链糖分子的 C-6 位 上，形成侧链，分子式为（C37H62O26）n。大麦水提多糖与 30 %乙醇提多糖的一级结构不同，使得两种多糖的降血糖作用有 区别。
Abstract:Study on the hypoglycemic effect of different structures of barley polysaccharide. Streptozotocin -induced diabetic mice model was built， in which the hypoglycemic effect of barley malt water extract， 30 % ethanol extract， 70 % ethanol extract was determined. The water extract and 30 % ethanol extract from barley malt decreased fasting plasma glucose of the diabetes mice and reduce blood glucose area under the curve. Further isolation of the water extract and 30 % ethanol extract， BP I was isolated from water extract by DEAE-cellulose，HW -55F and Sephacryl S -400. BP II was further isolated from 30 % ethanol extract by DEAE -cellulose，HW-55F and Sephacryl S-400. BP I consists only of glucofuranose units joined by 1→3 bond， and both of α and β configureation appear alternately. The main chain of BP II was galactoses linked by 1→4 bond， and the galactose， forming lateral chain， was linked to C-6 of the galactose in the main chain.
keywords: barley polysaccharides blood glucose glucose tolerance
文章编号：201602011 中图分类号： 文献标志码：A
基金项目:
| Author Name | Affiliation |
| HOU Yuan-yuan， YANG Yan-chao ， XU De-ping* | School of Food Science and Technology， Jiangnan University， Wuxi 214122， Jiangsu， China |
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