Abstract:To investigate the protective effect of Rhaponticum uniflorum extracts on liver injury induced by D-galactoamine（GalN） in mice， mice were randomly assigned to the normal control， model， bifendate， Rhaponticum uniflorum ethanol extract， as well as the butanol （RUBF） and aqueous fractions of Rhaponticum uniflorum. Animals were treated once daily for 7 days. At the end of the experiment， GalN was given intraperitoneally to the mice of groups， then the alanine aminotransferase （ALT）， aspartate aminotransferase （AST）， inducible nitric oxide synthase （iNOS）， nitric oxide （NO）， malondialdehyde （MDA）， glutathione （GSH）， glutathione peroxidase （GPx） and superoxide dismutase （SOD） were detected with the colorimetric method. The results showed that the administration with different solvent fractions of Rhaponticum uniflorum reduced the serum activities of ALT and AST （P＜0.05）， decreased hepatic iNOS and NO level， decreased the MDA content and increased GPx， SOD and GSH levels of liver mitochondria in mice with acute liver injury （P＜0.05）. This study showed that Rhaponticum uniflorum extracts had protective effects on acute liver injury induced by GalN in mice， probably via anti-oxidative and anti-inflammatory activities.