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投稿时间:2015-09-11
投稿时间:2015-09-11
中文摘要: 对干木薯渣进行物理粉碎,再用 α-淀粉酶和糖化酶(中温淀粉酶 60 ℃~70 ℃)、脂肪酶和风味蛋白酶除去木薯 渣中的淀粉、脂肪和蛋白质,得到纯净的木薯膳食纤维并用超声波辅助脱色;通过单因素试验得到了木薯渣化学成分去 除率和膳食纤维脱色的最佳工艺条件。化学成分去除的最佳工艺条件为:当 α-淀粉酶和糖化酶的质量比 1 ∶ 6,用量为0.6 %,酶解 pH=7,酶解时间为 120 min,酶解温度为 60 ℃时,淀粉去除率最高;当脂肪酶用量 0.21 %,酶解 pH=7,酶解时 间 90 min,酶解温度为 50 ℃时,脂肪去除率最高;当风味蛋白酶用量 0.6 %,酶解 pH=4,酶解时间 150 min,酶解温度为35 ℃时,蛋白质去除率最好。脱色的最佳试验条件为:当 H2O2 浓度为 10 %,漂白时间 40 min,超声功率为 60 W,漂白温 度 50 ℃时,漂白效果最好。
Abstract:Dry cassava residues were physically crushed, and then the starch, fat and protein of cassava residue were hydrolyzed by α-amylase and glucoamylase (medium temperature amylase 60 ℃ to 70 ℃), lipase and flavorzyme. Finally cassava dietary fiber was got and bleached it with the help of ultrasonic; the optimum technological conditions were confirmed through the single factor experiments. Experimental results showed that: when α-amylase and glucoamylase ratio was 1 ∶ 6, the concentration of them was 0.6 %, pH=7, enzymolysis time was 120 min, enzymolysis temperature was 60 ℃, starch removal rate was best; when lipase dosage was 0.21 %, pH=7, enzymolysis time was 90 min, enzymolysis temperature was 50 ℃, fat removal rate was best; when the concentration of flavourzyme was 0.6 %, pH=4, enzymolysis time was 150 min, enzymolysis temperature was 35 ℃, protein removal rate was best. When H2O2 concentration was 10 %, the bleaching time was 40 min, the ultrasonic power was 60 W, and the temperature was 50 ℃, the bleaching effect was best.
文章编号:201601026 中图分类号: 文献标志码:A
基金项目:现代农业产业技术体系专项基金(CARS-12);南科发[2014]
11 号重大专项资金
作者 | 单位 |
黄丽婕1,蔡园园1,古碧2,刘明1,王晓彤1,周雷1,杨莹3,劳彩娴1 | 1. 广西大学 轻工与食品工程学院,广西 南宁 530004;2. 广西大学淀粉研究所,广西 南宁 530004; 3. 广西清洁化制浆造纸与污染控制重点实验室,广西 南宁 530004 |
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