Abstract:Through the establishment of different exercise intensity treadmill exercise in aged rats model to explore the impact of GBE on antioxidant effect of different exercise intensity in aged rats tissue in this article. 56 aged rats had been divided into the drug group and the control group by random grouping method in two large groups. Rats in drug group and control group were divided into low intensity exercise group， the moderate intensity exercise group ， the large intensity exercise group and quiet group respectively. The malondialdehyde （MDA） content， CAT （catalase）， SOD （superoxide dismutase enzyme） and GSH-Px （glutathione peroxidase） enzyme activity had been detected in spleen and thymus to verify antioxidant effect of GBE in aged rats tissue. The results showed that GBE could effectively improve the body's antioxidant capacity and appropriate intensity and moderate intensity exercise could improve the body's antioxidant activity， but in the high strength state， the body's antioxidant activity would been decreased.