Abstract:To investigate the effects of inulin on animal immune function，search databases such as Medline，Embase，and China National Knowledge Infrastructure （CNKI） to collect randomized controlled trials related to inulin and immune function. The search period was from January 2000 to December 2023，resulting in 20 studies. Meta-analysis was conducted using Stata 18.0 software on nine immune indicators，including thymus index，spleen index，immunoglobulin A（IgA），immunoglobulin G（IgG），immunoglobulin M（IgM），etc. Sensitivity analysis and publication bias analysis were also conducted. The results indicated that the thymus index，spleen index，and levels of IgG，IgM，and IgA in the blood of the inulin experimental group were higher than those of the control group，and the proportion of CD4+T cells also increased，with statistically significant difference （P<0.05）. There was no statistically significant difference （P>0.05） in the CD4+/CD8+ratio，CD8+T cell proportion，and natural killer cell proportion in the blood between the two groups. Sensitivity analysis showed that the results for five indicators，including IgA and IgM，were relatively reliable. Additionally，There was no significant publication bias for six indicators including thymus index，spleen index，IgG，etc. The above results indicate that inulin can promote the development of animal immune organs，increase immunoglobulin content，and enhance the immune function of animal bodies to a certain extent，but more high-quality and large-scale studies are still needed for verification.