Abstract:This study established a streptozotocin （STZ）-induced type 1 diabetes mouse model. Seventy-two mice were divided into six groups：normal group，model group，acarbose group，low-dose formula group（625 mg/kg），medium-dose formula group （1 250mg/kg） and high-dose formula group （2500mg/kg）. After 4h of fasting，corresponding samples and starch solution were intragastrically administrated to conduct oral glucose tolerance tests. Meanwhile，a type 2 diabetes model was built by high-fat diet feeding combined with STZ injection. Another72mice were assigned to normal group，model group，metformin group，low-dose （100mg/kg），medium-dose （200mg/kg） and high-dose （400mg/kg） formula groups. Relevant phenotypic indicators were detected after 4 weeks of continuous gavage administration.The results showed that the medium and high doses of the formula significantly reduced postprandial blood glucose in type 1 diabetic mice. Compared with the untreated type 2 diabetic model mice，food intake decreased in all formula dose groups. Liver weight decreased significantly in all treatment groups，while the activities of superoxide dismutase and hexokinase increased，and serum insulin levels recovered in the high-dose group. Pathological observations demonstrated that liver lipid deposition，enlarged epididymal adipocytes and pancreatic islet atrophy were alleviated in all formula intervention groups. In conclusion，the instant powder mixed with mulberry leaf and Cyclocarya paliurus significantly lowered postprandial blood glucose and ameliorated insulin resistance in diabetic mice.