Abstract:Objective: To establish a novel analytical method for the simultaneous determination of eight biogenic amines in fermented alcoholic beverages by combining dansyl chloride derivatization with high-performance liquid chromatography-fluorescence detection (HPLC-FLD) and the quantitative analysis of multi-components by single marker (QAMS) strategy. Methods: Using tyramine as the internal reference substance, the relative correction factors for the remaining biogenic amines were established, and their robustness and reproducibility were systematically validated under different chromatographic conditions and instrument systems. Results: The correction factors for all components showed good stability, and there was no significant difference between the results obtained by the QAMS method and those by the conventional external standard method. Bland-Altman analysis confirmed a high degree of consistency between the two methods. Conclusion: A new QAMS method based on derivatization HPLC-FLD was successfully developed. This method enables the simultaneous quantification of eight biogenic amines using only one reference standard, significantly reducing detection costs and dependence on reference standards, and provides a practical and cost-effective solution for high-throughput quality control of biogenic amines in fermented foods.