Abstract:Aging is the primary risk factor for the occurrence of numerous diseases, and investigating the aging process holds profound significance for maintaining human health; neurological impairment is a common phenomenon in aging, as brain structure and function degenerate with age, neural cell vitality weakens, and conduction efficiency decreases, which not only affects cognitive abilities but also easily triggers health issues such as neurodegenerative diseases; the D-galactose-induced in vitro PC12 cell senescence model was adopted to explore the improvement effects of black rice anthocyanins (BRA) and lutein esters (LE) individually or in combination on the cell senescence model, preliminarily evaluating the anti-aging capabilities of BRA and LE based on cell viability and cellular oxidative stress levels; the results showed that within the concentration range of 5-120 μg/mL, both BRA and LE significantly improved the survival rate of D-gal-induced oxidative damage in PC12 cells, with their ED50 values being 7 μg/mL and 12 μg/mL respectively; both BRA and LE at half their ED50 concentrations individually mitigated the increase in ROS content caused by oxidative damage, and LE significantly reduced MDA content; the combination of BRA and LE at concentrations of 1/2 and 1/4 ED50 demonstrated synergistic effects, with the 1/2 concentration showing more pronounced results; compared to individual treatments, the combined use of BRA (3.5 μg/mL) and LE (6 μg/mL) reduced intracellular ROS and MDA levels, exhibiting significant improvement effects on cellular oxidative damage.