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投稿时间:2026-06-09 修订日期:2026-08-02
投稿时间:2026-06-09 修订日期:2026-08-02
中文摘要: 为解决葛根饮料易浑浊沉淀以及红茶“冷后浑”导致体系不稳定的问题,本研究以浮梁红茶为主料,通过双酶解、超声复合酶解和三元复合酶解的比较优化葛根糖化的复合酶比例,以茶多酚、葛根素为指标,确定红茶茶汤稀释度,在此基础上开发两款以浮梁红茶和葛根为基底的复合茶饮,并进行感官、功能成分和微生物安全性评价。结果表明,α-淀粉酶、果胶酶、糖化酶的最优配比为5∶3∶2,该条件下葛根还原糖得率达67.05 mg/mL,较传统双酶解提升95.9%,较文献水平提升90%以上;红茶茶汤4℃避光保存14 d无沉淀;茶多酚保留800 mg/L,葛根素保留率92%,符合药食同源茶饮产品特征。两款新产品感官接受度分别为84.35分和81.30分,其中葛根山楂洛神花饮更受女性青睐;产品ABTS自由基清除率均超90%,葛根素含量分别为0.10 mg/mL和0.11 mg/mL,微生物指标符合GB 7101-2022要求,巴氏灭菌后常温避光储存21 d稳定性良好。本研究证实三元复合酶解可显著改善葛根糖化效率和饮料稳定性,所研发的茶饮兼具良好感官接受度与抗氧化活性,为药食同源复配饮品提供了可参考的开发路径,也为浮梁特色农产品增值与茶旅融合提供了数据支撑。
Abstract:To address the turbidity and precipitation issues in Pueraria lobata beverages and the instability caused by the cold-induced turbidity of black tea, this study used Fuliang black tea as the main raw material and compared dual-enzyme, ultrasonic-assisted composite, and ternary composite enzymatic hydrolysis to optimize the enzyme ratio for Pueraria lobata saccharification. The dilution of black tea infusion was determined based on the retention of tea polyphenols and puerarin, and two compound tea beverages were developed and evaluated for sensory quality, functional components, and microbiological safety. The results showed that the optimal ratio of α-amylase, pectinase, and glucoamylase was 5:3:2, yielding a reducing sugar content of 67.05 mg/mL from Pueraria lobata, which was 95.9% higher than that of traditional dual-enzyme hydrolysis and over 90% higher than reported in the literature. The black tea infusion showed no precipitation after 14 days at 4?°C in the dark, with tea polyphenols retained at 800 mg/L and a puerarin retention rate of 92%, meeting the characteristics of medicine-food homology beverages. The two new products received sensory acceptance scores of 84.35 and 81.30, with the Pueraria lobata-hawthorn-hibiscus drink being more preferred by females. Both products exhibited over 90% ABTS free radical scavenging activity, with puerarin contents of 0.10 and 0.11 mg/mL, respectively. Microbiological indices met the requirements of GB 7101-2022, and the products remained stable after pasteurization and storage at room temperature in the dark for 21 days. In conclusion, ternary composite enzymatic hydrolysis significantly improves saccharification efficiency and beverage stability, and the developed products possess good sensory acceptability and antioxidant activity, providing a reference development pathway for medicine-food homology compounded beverages and data support for the value addition of Fuliang specialty agricultural products and tea = tourism integration.
文章编号:QY20260535 中图分类号: 文献标志码:
基金项目:冷链食品劣变化学标志物筛选方法及实时检测技术研究(2022YFF1100901);“十四五”重点研发专项,食品营养与安全关键技术研发);荻湾特色产品开发项目(202505510610064)
| 作者 | 单位 | 邮编 |
| 谭心悦 | 中国农业大学食品科学与营养工程学院 | 100083 |
| 张美懿 | 中国农业大学食品科学与营养工程学院 | 100083 |
| 李璐晴 | 中国农业大学食品科学与营养工程学院 | 100083 |
| 李家桐 | 中国农业大学食品科学与营养工程学院 | 100083 |
| 梁志宏* | 中国农业大学食品科学与营养工程学院 | 100083 |
| Author Name | Affiliation | Postcode |
| Tan Xinyue | 100083 | |
| 100083 | ||
| 100083 | ||
| 100083 | ||
| 100083 |
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