Abstract:The ultrasound-assisted alkali extraction and acid precipitation method was used to extract proteins from Zanthoxylum bungeanum leaves. The extraction process parameters （pH value， ultrasonic time， solid-toliquid ratio， and ultrasonic temperature） were optimized by single factor and orthogonal tests， and the functional properties of the extracted proteins were characterized. The results showed that the optimal extraction conditions were as follows： pH13.0， solid-to-liquid ratio of 1∶40 （g/mL）， ultrasonic power of 200 W， ultrasonic temperature of 45 ℃， ultrasonic time of 90 min， and pulsed ultrasound working interval ratio of 5∶3. Under these conditions， the recovery rate of proteins from Z. bungeanum leaves reached （54.96±1.29）%， which was 100.66% higher than that of constant temperature stirring extraction. The proteins obtained by ultrasoundassisted extraction from Z. bungeanum leaves had better solubility， oil holding capacity， foaming capacity，emulsifying activity， and emulsifying stability but lower water holding capacity and foaming stability than those by constant temperature stirring extraction. The proteins obtained by ultrasound-assisted extraction from Z. bungeanum leaves were fibrous， with the content of α-helixes decreasing from 23.66% to 22.55%， the content of random coils decreasing from 19.25% to 12.89%， and the content of β-sheets and β-turns increasing.