Abstract:The uncertainty in the development process of clothianidin matrix standard substance in ginger powder was evaluated through mathematical models from five aspects：uniformity，short-term stability，long-term stability，cooperative fixed value A，and cooperative fixed value B. The results indicated that short-term stability had the highest contribution （45.0%） to the total uncertainty，followed by long-term stability （24.4%） and uniformity （16.4%）. The cooperative fixed values A and B showed the contribution rates of 3.6% and 10.6%，respectively. In the development process of matrix standard substances，standardized operations should be carried out to ensure uniformity. During transportation，cold chain transportation should be adopted to ensure shortterm stability. During storage，attention should be paid to collection to ensure long-term stability. The characteristic value of this matrix standard substance was（0.109±0.006） mg/kg，which was suitable for method confirmation and quality control of clothianidin in agricultural products，helping to improve the accuracy of detection results and providing reference for uncertainty assessment of matrix standard substances related to agricultural products.