Abstract:This study aimed to explore the effect of low-voltage electrostatic field technology on the quality of Cynoglossus semilaevis during micro-freezing storage. By analyzing the freshness index and protein characteristic index of Cynoglossus semilaevis，the study investigated the effect of different （0，2 000，and 2 500 V/m） low voltage electrostatic field treatment on the muscle quality of Cynoglossus semilaevis. The results indicated that，with the extension of storage time，the myofibrillar protein content，calcium-dependent adenosine triphosphatase（Ca2+-ATPase) activity，total sulfhydryl content，and solubility showed a decreasing trend in muscle samples of all groups，while the low-voltage electrostatic field group demonstrated relatively ideal preservation of these indices. Conversely，the protein surface hydrophobicity，carbonyl content，and protein solubility exhibited an increasing trend in all groups，with the above three items being significantly higher in the control group.Sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE） results showed that at 30 days of storage，protein degradation occurred in the muscle of all groups，with the degree of protein degradation being significantly lower in the muscle of the 2 500 V/m low-voltage electrostatic field group. Microstructure observation of muscle showed that at 30 days of storage，the muscle structure of the low-voltage electrostatic field group samples was relatively ideal. In conclusion，the low-voltage electrostatic field technology could delay the muscle quality deterioration of Cynoglossus semilaevis.