Abstract:To study the purification process and adsorption mechanism of phloretin on macroporous adsorption resin，five kinds of macroporous adsorption resins on phloretin in apple blossom were compared respectively in terms of their adsorption and desorption performance. The best adsorption resin for the separation and purification of phloretin was screened out. Based on this，the adsorption mechanism of phloretin by macroporous adsorption resins was investigated kinetically through methods of static，dynamic adsorption and isothermal adsorption，and the optimal adsorption and elution conditions were determined. The results showed that the NKA resin had the best adsorption effect. 250 mL of 8 mg/mL sample solution was adsorbed at a flow rate of 1.0 mL/min and a pH value of 5.0 and then desorbed easily with 200 mL of 60% ethanol. The purity of the sample was increased from 1.70% to 4.25%，over 2.5 times higher. It indicated that the purification of the phloretin in apple blossom by the NKA resin was greatly enriched with a rather satisfactory purification effect. The adsorption isotherms were fitted by Langmuir and Freundlich models to determine that the adsorption mechanism of phloretin on the macroporous adsorption resin was more consistent with the Freundlich model.