Abstract:To investigate the effect of different extraction methods of Poria cocos on the regulation of intestinal flora in mice， 130 KM mice were randomly divided into a negative control group， low-dose and high-dose groups of raw P. cocos powder， low-dose and high-dose groups of cooked P. cocos powder， low-dose and highdose groups of P. cocos surrogate culture powder， low-dose， medium-dose， and high-dose groups of P. cocos water extract， and low-dose， medium-dose， and high-dose groups of P. cocos alcohol extract. Continuous gavage was administered for 7 d. After the final administration， fecal samples from the colon were collected for 16S rDNA sequencing. The results showed that the primary functions of P. cocos extracts obtained through various methods were associated with metabolism， genetic information processing， environmental information processing， cell transformation， and human disease-related genes. Compared with the negative control group， the P. cocos alcohol extract groups exhibited the highest α diversity index， and the high-dose P. cocos alcohol extract group showed the greatest number of operational taxonomic units （OTUs）， with Parasutterella （a genus in the phylum Proteobacteria） being the most abundant. In the low-dose raw P. cocos powder group， Akkermansia （a genus in the phylum Verrucomicrobiota） had the highest abundance. Different extraction methods of P.cocos could regulate the intestinal flora of mice and suggested potential improvements in the treatment of obesity and related metabolic diseases.