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投稿时间：2024-01-08
投稿时间：2024-01-08
中文摘要: 为研究常见果皮中游离酚、酯溶性酚和难溶性酚的含量及抗氧化活性情况，以11 种果皮为原料提取其中的游离酚、酯溶性酚和难溶性酚，测定11 种果皮的铁离子抗氧化能力（ferric ion reducing antioxidant power，FRAP）、DPPH 自由基清除能力、ABTS+自由基清除能力、总多酚含量，使用超高效液相色谱（ultra performance liquid chromatography，UPLC）仪对果皮提取物中的多酚进行定性和定量分析。结果表明，11 种果皮中游离酚含量为2.69～20.09 mg GAE/g DW，酯溶性酚含量为2.26～6.38 mg GAE/g DW，难溶性酚含量为0.72～13.41 mg GAE/g DW，总酚含量为9.03～27.88 mg GAE/g DW；游离酚、酯溶性酚以及难溶性酚的ABTS+自由基清除能力分别为29.03～273.48、29.97～144.91、36.65～195.03 μmol Trolox/g DW，DPPH 自由基清除能力分别为9.80～114.13、28.39～55.96、6.55～46.36 μmol Trolox/g DW，FRAP 分别为5.74～87.71、0.81～48.14、0.84～38.14 μmol Trolox/g DW。没食子酸、对香豆酸、芦丁、异槲皮苷、槲皮苷等广泛存在于11 种果皮中。综上，龙眼的多酚含量较高，抗氧化能力较强，是良好的抗氧化剂来源。
Abstract:To investigate the content and antioxidant capacity of free，esterified，and insoluble-bound phenolics in common fruit peels，this paper employed 11 types of fruit peels as the materials to extract free，esterified，and insoluble-bound phenolics from them. Meanwhile，the ferric ion reducing antioxidant power （FRAP），DPPH free radical scavenging power，ABTS+ free radical scavenging power，and total phenolic content of the fruit peels were determined. Additionally，qualitative and quantitative analyses were conducted on the phenolics in peel extracts by ultra-high performance liquid chromatography （UPLC）. The results showed that the free phenolic content in 11 types of fruit peels was 2.69-20.09 mg GAE/g DW；the esterified phenolic content was 2.26-6.38 mg GAE/g DW；the insoluble-bound phenolic content was 0.72-13.41 mg GAE/g DW；the total phenolic content was 9.03-27.88 mg GAE/g DW. The ABTS+ free radical scavenging power of free，esterified and insoluble-bound phenolics was 29.03-273.48，29.97-144.91，and 36.65-195.03 μmol Trolox/g DW respectively. The DPPH free radical scavenging power was 9.80-114.13，28.39-55.96，and 6.55-46.36 μmol Trolox/g DW respectively，with the FRAP of 5.74-87.71，0.81-48.14，and 0.84-38.14 μmol Trolox/g DW respectively. Gallic acid，coumaric acid，rutin，isoquercitrin，quercitrin，and other compounds were common in the 11 types of fruit peels. In summary，Dimocarpus longgan Lour. had a relatively high phenolic content and strong antioxidant activity，serving as a sound source of antioxidants.
keywords: fruit peels free phenolics esterified phenolics insoluble-bound phenolics antioxidant activity
文章编号：202510011 中图分类号： 文献标志码：
基金项目:广东省医学科研基金项目（B2021212、A2015617）；佛山市自筹经费类科技计划项目（1920001000268）；水乳类化妆品中植物精油抗菌效果的研究（0522270011）；仓储白砂糖变红原因调查（0523270016）；制剂型培养基在食品微生物检测行业的适用性研究（0522270005）
| 作者 | 单位 |
| 刘学文1，2，高裕锋1，2，骆春霞3，4，韩门娣3，4，谭强3，4，郭垚3，4，梁俭仪3，4，陈冠林3，4 * | 1.广东省科学院生物与医学工程研究所,广东 广州 510316；2. 国家轻工业甘蔗糖业质量监督检测中心,广东 广州 510316；3.佛山市职业病防治所,广东 佛山 528000；4.佛山市职业健康研究院,广东 佛山528000 |
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