Abstract:This paper employed the papain and neutral protease to hydrolyze hemp meal and thus obtain hemp peptides. Peptides with a relative molecular mass less than 1 000 Da were acquired by membrane separation technology. Meanwhile， the relative molecular weight distribution range of hemp peptides was determined by MALDI-TOF time-of-flight mass spectrometry. The effect of anti-liver cancer cells of hemp peptides in vivo was studied by building a human liver cancer Hep3B cell zebrafish tumor cell model. The results showed that the relative molecular weight of hemp peptides was mainly below 600 Da， thus inhibiting both the growth of tumor cells in zebrafish and the formation of subintestinal neovasculature. Pathological tissue slices revealed that hemp peptides could significantly reduce the number of tumor cells in the abdominal cavity. In conclusion，hemp peptides could be adopted as functional active substances to improve the life quality of patients with liver cancer.