Abstract:Cellulose acetate （CA）， an important material for food packaging， is hard to degrade in natural environments due to its high acetyl content. To enhance the degradability of CA， this study investigated the deacetylation effects of acid pretreatment， oxidation pretreatment， and alkali pretreatment on CA， as well as the enzymatic hydrolysis effects of single enzymes and compound enzymes on the corresponding deacetylated samples. The results showed that the NaOH-95% EtOH alkali pretreatment had the best deacetylation effect among the three pretreatment methods. After the samples with different degrees of substitution （DSs） were degraded by single esterase， the weight loss rate showed the same rule， that is， DS1.65 ＞ DS1.92 ＞ DS2.16. After the samples with different DSs were degraded by single cellulase for 196 h， the total sugar content of the DS1.65 sample was 28.46% （cellulase 1）， 14.35% （cellulase 2）， and 22.28% （cellulase 3）， respectively. The total sugar content of the DS1.92 sample increased slowly， while the total sugar content of the DS1.92 sample was very low. In the formula for compound enzyme hydrolysis， lipase PS with cellulase 1 and lipase A with cellulase 3 had the best hydrolysis effect. Compared with single cellulase hydrolysis， the above two formulas increased the total sugar content of DS1.65 by 9.64% and 15.82%， respectively， increased the total sugar content of DS1.92 by 1.52% and 1.89%， respectively， but had little effect on DS2.16 sample. Therefore， lipase compounded with cellulase can significantly improve the degradability of the samples with low DS.