本文已被：浏览 133次 下载 157次
投稿时间：2023-12-14
投稿时间：2023-12-14
中文摘要: 对野外采集的皱木耳开展真菌种属（internal transcribed spacer，ITS）测序及构建系统发育树鉴定菌株分支，并研究其菌丝最适温度、pH 值、无机盐浓度比及培养基最优配方等生物学特性。结果表明，检测序列与皱木耳Auricularia delicata 支持率达99%；菌丝培养最宜温度为25 ℃，最宜pH 值为6，无机盐PO43-和Mg2+浓度比为1.0%∶1%，正交结果显示最优配方为糊精粉10.0 g、乳糖5.0 g、牛肉膏2.0 g、酵母浸粉2.0 g、KH2PO4 1 g、MgSO4·7H2O 1 g、维生素B1 10 mg、琼脂20 g、去离子水1 000 mL。
Abstract:The internal transcribed spacer（ITS）sequencing and phylogenetic analysis were carried out to identify the Auricularia delicata samples collected in the field. The optimal temperature，pH value，inorganic salt concentration ratio，and medium formula for the mycelial growth were determined.The results showed that the sequence identity between the collected samples and A. delicata was 99%. The optimal culture conditions for mycelial growth were 25 ℃，pH6，and PO43-：Mg2+concentration ratio of 1.0%：1%.The orthogonal test results showed that the optimal formula was composed of 10.0 g dextrin powder，5.0 g lactose，2.0 g beef extract，2.0 g yeast extract，1 g KH2PO4，1 g MgSO4·7H2O，10 mg vitamin B1，20 g agar，and 1 000 mL deionized water.
文章编号：202419026 中图分类号： 文献标志码：
基金项目:贵州省科技计划项目（黔科合重大专项字［2019］3007-5 号、黔科合成果［2020］1Z003 号）；贵州省特色林业产业科研项目（贵［2022］TSLY06）
| Author Name | Affiliation |
| HE Yaowei，HUANG Jing，LI Peng，XIANG Zhun* | Guizhou Institute of Biology，Guiyang 550001，Guizhou，China |
引用文本：
津ICP备20000603号-1