Abstract:In this study，measures were taken to optimize the extraction process of Psoralea corylifolia glycoprotein（PCG），and to analyze its components. On the basis of single-factor experiment，PCG extraction rate was taken as the indicator to examine material liquid ratio，enzymatic hydrolysis time，and the amount of enzyme added.PCG′s molecular weight was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis（SDS-PAGE）. PCG′s components were determined by Fourier transform infrared spectroscopy（FTIR），ultra-high-speed automatic amino acid analyzer，and high performance liquid chromatography（HPLC）. The results showed that the PCG extraction rate was the highest with the addition of alkaline protease. The optimized extraction process for PCG was as follows：material liquid ratio was 1∶30（g/mL），enzymatic hydrolysis time was 2.5 h，and the amount of enzyme added was 3 000 U/g.The molecular weight of PCG extracted in the optimized process was 17-75 kDa. PCG contained amino acid and carbohydrate groups. Among 16 types of amino acids in the PCG，the content of glutamic acid was at 5.13%，the highest in all. Besides，there were 5 types of monosaccharides，with the content of mannose at 44.43%，the highest in all.