Abstract:This study explored the mechanism of Fu brick tea in regulating lipophagy for intervention in non-alcoholic fatty liver induced by high-fat and high-sugar diet in mice. Thirty-six male C57BL/6 micewere randomly divided into control，model，atorvastatin，and low-，medium-，and high-dose Fu brick tea groups.The control group was fed a normal diet，while the other five groups were fed a high-fat and high-sugar diet for 10 weeks after 1 week of acclimatization. The atorvastatin group received intragastric administration of atorvastatin at 10 mg/（kg·d），and the low-，medium-，and high-dose Fu brick tea groups received intragastric administration of Fu brick tea at 0.75，1.5，3.0 g/（kg·d），respectively.The control and model groups received intragastric administration of saline at 10 mL/（kg·d）. After 10 weeks of treatment，liver index，serum liver function，and blood lipids were detected，and hematoxylin and eosin（HE）staining，oil red O staining，transmission electron microscopy，immunofluorescence double-labeling，and western blot were performed. In the model group，extensive fatty vacuoles and severe lipid deposition were observed in liver tissues，indicating the successful establishment of the model.In the low-，medium-，and high-dose Fu brick tea groups，the extent of fatty vacuoles and lipid deposition in liver tissues decreased to varying degrees. The content encapsulated in autolysosome increased under electron microscopy，and liver pathological damage was improved. Liver index，abdominal fat mass，and the content of aspartate transaminase（AST），alanine aminotransferase（ALT），cholesterol（CHO），triglycerides（TG），low-density lipoprotein cholesterol（LDL-C），sequestosome-1（p62），phosphorylated phosphatidylinositol 3-kinase（p-PI3K），phosphorylated protein kinase B（p-AKT），and phosphorylated mammalian target of rapamycin（p-mTOR）were reduced to different extent（P<0.05 or P<0.01），while lipoprotein cholesterol（HDL-C），lysosome-associated membrane protein 2（LAMP2），microtubule-associated protein 1 light chain 3-II to microtubule-associated protein 1 light chain 3-I（LC3II/LC3I）ratio，and the co-localization degree of microtubule-associated protein 1 light chain 3 beta（LC3B）and perilipin-2（PLIN2）were increased to varying degrees（P<0.05 or P<0.01）.The high-dose Fu brick tea group showed better results than the low-dose Fu brick tea and atorvastatin groups，which exhibited a concentration-dependent effect. In conclusion，Fu brick tea can upregulate lipophagy levels，regulate lipid metabolism，and improve hepatic steatosis，which is possibly associated with the inhibition of the PI3K/AKT/mTOR pathway.