Abstract:Intestinal flora is a complex ecosystem in the human body closely related to human health. It plays an important role in various aspects. Overuse of antibiotics not only inhibits pathogenic bacteria but also may disrupt the normal probiotics population. To address the adverse effects of antibiotics on the gut microbiota，this study，treated an antibiotic-interference mouse model with low，medium，and high doses（1×106，1×107，1×108 CFU/mouse）of a compound lactic acid bacterium（Lactobacillus acidophilus LA-06，Bifidobacterium animalis subsp. lactis XLTG11，Lacticaseibacillus casei Glory LC18，and Lacticaseibacillus rhamnosus Glory LG12）.This study measured changes in mouse body weight，pathological structure，intestinal barrier，intestinal permeability，and short-chain fatty acids. The results showed that the compound lactic acid bacteria effectively increased the body weight of mice，promoted the growth of beneficial bacteria，maintained the integrity of intestinal tissue，enhanced the function of intestinal barrier，and reduced the permeability of intestinal barrier. The conclusion was that the compound lactic acid bacteria have an excellent probiotic function and could significantly improve the antibiotics-induced diarrhea in mice in a dose-dependent manner.