Abstract:This study aimed to explore the protective mechanism of Allium tenuissimum L. flower polysaccharide on alcohol-induced oxidative damage in mice. The in vitro antioxidant activity of A. tenuissimum L. flower polysaccharide was evaluated using the scavenging abilities against DPPH·， ABTS+·， hydroxyl radical （·OH），and total reducing power. An acute alcohol-induced liver injury animal model was constructed to measure the levels of liver injury markers and oxidative stress indicators in mouse serum and liver tissues. Liver pathology was observed by hematoxylin-eosin staining， and real-time quantitative polymerase chain reaction （qRT-PCR）was performed to measure the expression of oxidative stress pathway-related genes in liver tissues. The IC50 values of A. tenuissimum L. flower polysaccharide for DPPH·， ABTS+·， and ·OH were 0.79， 0.60， 1.26 mg/mL， respectively， but the total reducing power was relatively weak. Animal experiments showed that pre-administration of polysaccharide from Allium tenuissimum L. flower improved the mental state of mice， reduced the levels of phosphatase， transaminase， and harmful metabolites in serum， positively regulated the levels of inflammatory factors， enhanced antioxidant enzyme activity， restored the basic structure of liver tissue， and significantly reduced the infiltration of inflammatory factors. The qRT-PCR showed that the relative expression levels of Nrf2， GCLC， and HO-1 mRNA significantly increased， while Keap1 mRNA expression significantly decreased. A. tenuissimum L. flower polysaccharide had a significant protective effect on alcohol-induced oxidative injury in mice， which might be related to the activation of the Nrf2-mediated signaling pathway， providing a new therapeutic approach for alcoholic liver disease.