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食品研究与开发:2024,45(16):105-112
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桑枝低聚糖促枯草芽孢杆菌增殖工艺优化及其对产短链脂肪酸的作用
(1.华南农业大学 食品学院,广东 广州 510642;2. 广东省农业科学院 蚕业与农产品加工研究所,农业农村部功能食品重点实验室,广东省农产品加工重点实验室,广东 广州 510610;3. 广西五和博澳药业有限公司,广西 河池 546399)
Optimization of the Proliferation Process of Bacillus subtilis Promoted by Ramulus mori Oligosaccharides and Its Effect on the Production of Short-Chain Fatty Acids
(1. College of Food Science,South China Agricultural University,Guangzhou 510642,Guangdong,China;2. Sericultural & Agri-Food Research Institute,Guangdong Academy of Agricultural Sciences,Key Laboratory of Functional Foods,Ministry of Agriculture and Rural Affairs,Guangdong Key Laboratory of Agricultural Products Processing,Guangzhou 510610,Guangdong,China;3. Guangxi Wehandbio Pharmaceutical Co.,Ltd.,Hechi 546399,Guangxi,China)
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投稿时间:2023-08-31    
中文摘要: 为确定促进枯草芽孢杆菌增殖的最佳桑枝低聚糖酶解条件,以枯草芽孢杆菌增殖率为指标,利用单因素试验探究酶添加量、酶解温度和酶解时间对增殖率的影响,结合响应面法优化桑枝低聚糖的酶解工艺,并探究桑枝低聚糖对枯草芽孢杆菌产短链脂肪酸的影响。结果表明,最佳的桑枝低聚糖酶解工艺条件为酶添加量1 008 U/mL、酶解温度45 ℃、酶解时间4 h,该条件下枯草芽孢杆菌增殖率为(190.17±3.74)%。优于同等质量浓度下桑枝多糖对枯草芽孢杆菌的增殖率,此外桑枝低聚糖可以显著提高枯草芽孢杆菌产乙酸和丙酸的能力。桑枝低聚糖对枯草芽孢杆菌具有良好的促增殖潜力。
Abstract:To determine the optimal enzymatic conditions of Ramulus mori oligosaccharides promoting the proliferation of Bacillus subtilis,the proliferation rate of B. subtilis was used as the response value. A single-factor test was employed to investigate the effect of enzyme addition,enzymolysis temperature,and enzymolysis time on the proliferation rate. The response surface method was combined to explore the effect of R. mori oligosaccharides on the production of short-chain fatty acids by B. subtilis. The results showed that B. subtilis proliferation was best promoted by R. mori oligosaccharides enzymolysis technology when the enzyme addition was 1 008 U/mL,with the enzymolysis temperature of 45 ℃ and the enzymolysis time of 4 hours. The proliferation rate of B. subtilis was (190.17±3.74)% with these conditions,superior to that promoted by R. mori polysaccharide with the same concentration. In addition,R. mori oligosaccharides can significantly improve the ability of B. subtilis to produce acetic acid and propionic acid. R. mori oligosaccharides owned a good potential to promote B. subtilis proliferation.
文章编号:202416014     中图分类号:    文献标志码:
基金项目:广西科技计划项目(桂科AB21196040);广东省现代农业产业技术体系创新团队建设专项资金(2023KJ124、2023KJ117);广东省农业科学院农业优势产业学科团队建设项目(202119TD);国家蚕桑产业技术体系建设专项(CARS-18-ZJ0503);广东省农业科学院水产协同创新中心项目(XT202304、XT202234、202138)
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