本文已被：浏览 114次 下载 54次
投稿时间：2023-06-06
投稿时间：2023-06-06
中文摘要: 为探究羧甲基魔芋葡甘聚糖（carboxymethyl konjac glucomannan，CMKGM）在姜黄素结肠靶向递送体系构建中的应用潜力，以CMKGM 与卵清蛋白（ovalbumin，OVA）形成的聚电解质复合物为稳定剂制备姜黄素Pickering 乳液，研究两者质量比和总聚合物浓度对所得Pickering 乳液稳定性、外观结构、姜黄素包封率和载药量的影响，并对最佳乳液在模拟胃肠液消化过程中姜黄素释放率、乳滴粒径和形态的变化规律进行跟踪。结果表明，当OVA 与CMKGM 质量比为1∶1、总聚合物浓度为0.4%时所得Pickering 乳液的稳定性最好且姜黄素的包封率和载药量最高，分别达到了91.79%和1.05 mg/g。体外模拟消化研究表明，与OVA 稳定的姜黄素乳液相比，该Pickering 乳液在模拟胃液中具有更好的稳定性，且在模拟结肠液中的姜黄素释放率高达22.78%，远高于OVA 稳定乳液的5.35%，表现出了良好的结肠靶向递送性能。
Abstract:To explore the potential of carboxymethyl konjac glucomannan （CMKGM） in the construction of colon-targeted delivery systems for curcumin，the ovalbumin （OVA）-CMKGM polyelectrolyte complex was used as a stabilizer to fabricate curcumin-loaded Pickering emulsion. The effects of the mass ratio of OVA and CMKGM as well as total biopolymer concentration on the stability，appearance，curcumin encapsulation efficiency，and drug loading were investigated. The curcumin release，particle size variation，and morphology variation patterns of the optimum emulsion in the simulated gastrointestinal fluids were tracked. The results indicated that the OVA to CMKGM mass ratio of 1∶1 and total biopolymer concentration of 0.4% conferred the highest curcumin encapsulation efficiency and drug loading of 91.79% and 1.05 mg/g respectively. In vitro simulated digestion tests revealed that compared with the curcumin emulsion stabilized by OVA，the Pickering emulsion exhibited better stability in the simulated gastric fluid. Its curcumin release rate was up to 22.78% in the simulated colonic fluid，much higher than that of the OVA-stabilized emulsion （5.35%），validating its excellent colon-targeted delivery performance.
keywords: curcumin carboxymethyl konjac glucomannan ovalbumin polyelectrolyte complex colonic delivery system Pickering emulsion
文章编号：202416006 中图分类号： 文献标志码：
基金项目:国家级、省级大学生创新创业训练计划项目（202210435042）
引用文本：
津ICP备20000603号-1