Abstract:To study the anti-alcohol and liver protection effects of Pueraria lobata extracts before and after fermentation，this paper conducted an experiment to build a model of alcoholic-induced liver injury in rats with alcoholic gavage of 56% vol alcohol level and 13 mL/kg.There were several groups including the blank group，model group，positive group（0.945 g/kg），pre-fermentation extract group（1.6 g/kg），and post-fermentation extract group（1.6 g/kg）.Meanwhile，gavage administration was carried out based on the group classification.The effects of Pueraria lobata extracts on alcohol tolerance，and sleep and waking time of rats before and after fermentation were investigated.Additionally，this study also investigated the influences of alanine aminotransferase（ALT），aspartate aminotransferase（AST），triglycerides（TG）levels in rat serum，alcohol dehydrogenase（ADH），superoxide dismutase（SOD），glutathione peroxidase（GSH-Px）activities，and malondialdehyde（MDA）level in rat liver tissues.Meanwhile，the changes in rat liver status were observed by hematoxylineosin（HE）staining of tissue sections.The results showed that compared with the model group，the Pueraria lobata extract group had anti-alcohol and liver protection effects before and after the fermentation.Further，in comparison with the pre-fermentation group，the post-fermentation group could prolong the alcohol tolerance，and shorten sleep and waking time（P＜0.05）.Meanwhile，this group could also reduce the AST，ALT，and TG levels（P＜0.001），and enhance ADH，SOD，and GSH-Px activities（P＜0.01）in liver tissues，with a more obvious decrease in the MDA level（P＜0.01）.The HE staining results indicated that the steatosis degree in hepatocyte steatosis was lower in the post-fermentation group than in the pre-fermentation group.Pueraria lobata extracts had anti-drunkenness，anti-alcoholic，and liver protection effects.Meanwhile，the effects of Pueraria lobata extracts after fermentation were much better than those before fermentation.