Abstract:This paper investigates the inhibitory and clearance effects of postbiotic Probio-Eco on Helicobacter pylori（Hp），the effects on Hp adhesion to human gastric adenocarcinoma cell line AGS，as well as evaluates and analyzes the ability of Probio-Eco to reduce the secretion of the inflammatory factor interleukin-8（IL-8）by AGS induced by Hp.The results showed that Probio-Eco could effectively inhibit the growth of Hp and reduces Hp adhesion to human gastric adenocarcinoma cell line AGS [the adhesion rates of Hp bio-73976，Hp bio-73975，and Hp bio-73974 were（74.25±6.13）%，（79.92±4.36）%，and（72.13±5.83）%，respectively]，and it could inhibit Hp-induced secretion of the inflammatory factor IL-8 by gastric epithelial cells [the inhibition rates of Hp bio-73976，Hp bio-73975，and Hp bio-73974 were（685.23±21.34），（632.75±19.35），and（690.32±18.43）pg/mL，respectively]，all of which differed significantly（P＜0.01）when compared with the model group.The interagglutination between Probio-Eco and Hp was enhanced with the prolongation of the standing time，and the interagglutination rate reached more than 90.0% after 24 h of standing.The electron microscope results revealed that Probio-Eco caused Hp to show obvious morphological and structural changes such as indentation and rupture.In conclusion，Probio-Eco can effectively inhibit the growth of Hp，reduce the adhesion rate of Hp to human gastric adenocarcinoma cell，inhibit the secretion of inflammatory factors by human gastric adenocarcinoma cell induced by Hp，and it can lead to morphological and structural changes of Hp such as indentation and rupture.