Abstract:Glucose oxidase（GOD），as an aerobic dehydrogenase，can specifically oxidize β-D-glucose to hydrogen peroxide and gluconic acid by using oxygen.Based on this mechanism，glucose oxidase shows bactericidal and antibacterial activity，which is widely used in commercial fields such as food，feed，clinical diagnosis and biomonitoring.However，the application of GOD is various in different fields，so it is necessary to use advanced biotechnology to optimize the characteristics of GOD and develop targeted enzyme preparations according to different characteristics of GOD.This paper summarizes the source，properties，heterologous expression and multi-field application of glucose oxidase，in order to provide new insights for the development and industrial application of glucose oxidase.