Abstract:In this paper，ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry（UPLC-Q/TOF-MS）was utilized to collect the entire metabolite profiles of collagen peptide solution（PP），non-fermented jackfruit juice（JJ），fermented jackfruit juice（FJJ），and co-fermented peptide-jackfruit juice（FPJ），so as to compare the metabolite differences between the FPJ group and FJJ，JJ，PP groups.A total of 1 388 metabolite features were identified in positive ion mode and 565 metabolite features were identified in positive and negative ion mode.Using a threshold of VIP>1.0 and Fold Change>2 and metabolomics databases for precise identification，80 metabolites were screened out as key metabolites，including 42 oligopeptides，7 alcohols，6 amino acids，3 organic acids，3 esters，3 lipids，2 aldehydes，etc.The results showed that the FPJ group exhibited a significant change in the contents of amino acids，small peptides，esters，acids，and flavor compounds compared with the FJJ and JJ groups，thereby enhancing the nutritional value and sensory quality of the FPJ group.