Abstract:This paper aimed to screen an oligonucleotide sequence that was complementary to the self-quenching fluorogenic probe，and construct a visualization system using the fluorescence super-quenching mechanism of double-stranded DNA，to realize the rapid visual detection of loop-mediated isothermal amplification（LAMP）based on the self-quenching fluorogenic probe. The results showed that the background fluorescence intensity of the system was decreased after the addition of an oligonucleotide sequence that was completely complementary to the self-quenching fluorogenic probe（P＜0.05）. When the melting temperature（Tm）was higher than room temperature，the quenching effect was not impacted by shortening the length of the complementary sequence（from the 3′ end）. The LAMP visualization system，based on the fluorescence superquenching mechanism of the complementary sequence，effectively reduced the background fluorescence and provided easy-to-read visualization results after reaction. It has been successfully used for rapid detection of Oncorhynchus mykiss.In summary，an appropriate complementary sequence would reduce the background fluorescence of the self-quenching fluorogenic probe，and it was applicable to establish a low-background fluorescence LAMP system by combining this complementary sequence with the self-quenching fluorogenic probe，which was of certain promotional value.