Abstract:This paper investigated the effect of essential oil from Elsholtiza bodinieri vaniot on adipogenic differentiation and lipid-reducing mechanism of mouse embryonic mesenchymal stem cells C3H10T1/2. The survival of cells was measured by the 3-（4，5-dimethylthiazol-2-yl）-2，5-diphenyltetrazolium bromide（MTT）method to define the appropriate range of concentration. After treatment with E. bodinieri essential oil，the oil red O staining was performed to analyze the cell adipogenic differentiation.3-（4，5-dimethylthiazol-2-yl）-2，5-diphenyltetrazolium bromide（GPO-PAP），and cholesterol oxidase，peroxidase and 4-aminoantipyrine phenol（COD-PAP）were used to detect the changes in triglyceride（TG）and total cholesterol（TC）in cells treated with essential oil. The mRNA and protein expressions of peroxide proliferator-activated receptor γ（PPARγ），CCAAT/enhancer-binding proteins α（C/EBPα），and fatty acid-binding protein 4（FABP4）in lipid differentiation were detected by quantitative polymerase chain reaction（PCR）and western blot，respectively. The results showed that compared with the normal blank control group，the high-fat model group had increased TC and TG（P＜0.05），and the mRNA and protein expressions of PPARγ，C/EBPα，and FABP4 in C3H10T1/2 cells were up-regulated.Compared with the conditions in high-fat model group，the TC and TG in middle-dose（50 μg/mL）and high-dose（100 μg/mL）essential oil group were decreased，and the mRNA and protein expressions of PPARγ，C/EBPα，and FABP4 in C3H10T1/2 cells were down-regulated. The results indicated that the essential oil may inhibit the adipogenic differentiation of C3H10T1/2 cells by regulating PPARγ/C/EBPα/FABP4 signaling pathway to achieve lipid reduction.