Abstract:This paper explored the effect of whey protein and soybean protein on bone mineral density in rats.The model of low bone density was prepared by removing the ovaries of rats. They were divided into sham operation group,model group,dual-protein group（10 g/kg）,estradiol benzoate group（0.2 mg/kg）and calcium carbonate group（1.3 g/kg）. After continuous gavage for 90 d,the rats were sacrificed and their femurs were separated. Bone calcium content was measured by atomic absorption spectrometry,and bone mineral density was measured by bone densitometer. The results showed that compared with the conditions in sham operation group,the bone mineral density and bone calcium content in the model group were decreased（P<0.01,P<0.05）.Compared with the model group,the dual-protein group had increased midpoint bone mineral density（P<0.05）,increased bone mineral density at the distal end（P<0.01）,and significantly improved femurs.The bone mineral density and bone calcium content in the estradiol benzoate group were higher（P<0.01）,and the bone mineral density at the distal end in the calcium carbonate group was also higher（P<0.01）than those in the model group.Dual-protein had the effect of increasing bone mineral density in ovariectomized rats.