Abstract:This study was conducted to explore the effects of probiotic fermented tomato juice（PFTJ）on antifatigue and intestinal microflora in mice. Forty male ICR mice were randomly divided into four groups:blank control group,positive control group（0.06 g/kg）,low-dose and high-dose PFTJ groups（5 and 15 mg/kg）.The anti-fatigue effect of PFTJ was assessed by load-bearing swimming exhaustion time,biochemistry indicators and the expression of genes related to energy metabolism.Furthermore,the composition and structure of intestinal microflora were analyzed.The results showed that high-dose PFTJ could significantly prolong the swimming time under load exhaustion,lower the levels of blood urea nitrogen（BUN）,serum lactic acid（LA）and malondialdehyde（MDA）,as well as increase the levels of muscle glycogen,superoxide dismutase（SOD）and glutathione peroxidase（GSH-PX）in comparison with the blank control group.Additionally,PFTJ significantly up-regulated the mRNA expression levels of AMPK and PGC-1α in mouse skeletal muscles. PFTJ also increased the diversity of intestinal microflora. The abundance of Lɑctobɑcillus and norɑnk_f_Muribɑculɑceɑe was up-regulated,while the proportion of Erysipelotrichɑceɑe was down-regulated.The results suggest that PFTJ has potential anti-fatigue ability,which is directly associated with decreasing metabolites accumulation,inhibiting glycogen depletion,antioxidation and activating AMPK/ PGC-1α signaling pathway. The anti-fatigue activities of PFTJ may be indirectly achieved by regulating the community structure of intestinal microflora in mice.