Abstract:The preservation effect of space electric field on Trichiurus lepturus was studided. High-throughput sequencing（Illumina Miseq）was employed to determine the microbial changes and base segments of Trichiurus lepturus collected in Zhoushan and preserved by space electric field combined with micro-freezing preservation. Operational taxonomic unit（OTU）clustering and correlation analysis，diversity analysis，microbial composition analysis，microbial community structure analysis，and correlation analysis were conducted to reveal the microbial community composition and biological information that affect the preservation effect of T. lepturus. The electric fields were set at 3 kV/m and 2 kV/m，respectively，and the non-electric field treatment group was taken as the control. Each group of samples was stored at -4 ℃. The effects of electric field treatments at different intensities on the microbial community composition of T. lepturus were compared among the three groups. The microbial relative abundance suggested that during the preservation process of T. lepturus under the combination of space electric field and low temperature，the effects of Psychrobacter，Pseudomonas，and Janthinobacterium on the spoilage of T. lepturus were significant. After 20 days，the relative abundance of OTU6（Psychrobacter）and OTU13（Pseudomonas）in the samples treated with 3 kV/m electric field was less than 1%，and OTU7，OTU14，OTU8，and OTU9 were not clustered. The results indicated that there were hygiene quality risks in the preservation process of T. lepturus，and space electric fields can inhibit the proliferation of spoilage bacteria in T. lepturus，thereby extending its shelf life.