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食品研究与开发:2024,45(4):127-133
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黑青稞γ-氨基丁酸提取工艺优化及体外降血糖活性
(1.青海大学农牧学院,青海西宁 810016;2.青海大学省部共建三江源生态与高原农牧业国家重点实验室,青海西宁 810016)
Extraction Process Optimization of γ-Aminobutyric Acid in Black Highland Barley and Its Hypoglycemic Activity in vitro
(1.College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,Qinghai,China;2.State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University,Xining 810016,Qinghai,China)
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投稿时间:2022-11-11    
中文摘要: 该试验以黑青稞为原料,以γ-氨基丁酸(γ-aminobutyric acid,GABA)含量为响应指标,探究提取温度、提取时间、液料比对GABA 含量的影响,并进行体外降血糖活性研究。在单因素试验的基础上,将3 个影响因素进行响应面试验,试验结果表明黑青稞提取GABA 最佳工艺参数为提取温度60 ℃、提取时间90 min、液料比3∶1(mL/g),在该条件下,黑青稞中GABA 含量为(0.338±0.012)mg/mL,与模型理论值0.341 mg/mL 接近,说明响应面模型可较好地优化黑青稞GABA 的提取工艺。黑青稞中GABA 对α-淀粉酶的抑制率为(72.41±0.43)%,对α-葡萄糖苷酶抑制率为(82.29±0.38)%,表明其具有较好的体外降血糖效果。
Abstract:With black highland barley as the raw material and the content of γ-aminobutyric acid(GABA)as the response index,this paper analyzed the influence of extraction temperature,extraction time and liquidmaterial ratio on GABA content,and explored the hypoglycemic activity in vitro. On the basis of single factor test,the three influencing factors were tested by response surface methodology.The results showed that the optimal extraction process parameters of GABA in black highland barley were as follows:extraction temperature 60 ℃,extraction time 90 min,and liquid-material ratio 3∶1(mL/g). Under these conditions,the GABA content in black highland barley was(0.338±0.012)mg/mL,which was close to the model predicted value of 0.341 mg/mL,indicating that the response surface model optimized the extraction process of GABA in black highland barley.The inhibition rate of GABA in black highland barley on α-amyase was(72.41±0.43)%,and that of GABA on α-glucosidase was(82.29±0.38)%,suggesting a good hypoglycemic activity in vitro.
文章编号:202404017     中图分类号:    文献标志码:
基金项目:青海省科技厅自然科学基金青年项目(2020-ZJ-975Q);省部共建三江源生态与高原农牧业国家重点实验室自主课题项目(2019-ZZ-03)
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