Abstract:The changes of active components and antioxidant activity of Aronia melanocarpa extract during simulated gastrointestinal digestion in vitro were explored. The content of flavonoids，polyphenols，anthocyanins，and procyanidin in simulated oral，gastric，and intestinal digestive fluids and intestinal dialysate of A.melanocarpa extract and in A. melanocarpa extract was determined by ultraviolet spectrometry. Furthermore，the hydroxyl，DPPH，ABTS+，and superanoxide free radical scavenging activities of these active components were determined. The results showed that procyanidin had the highest content[（333.86±3.46）mg/g]in the A.melanocarpa extract，followed by polyphenols，and anthocyanins had the lowest content.The active components in samples changed differently during the simulated digestion. The content of anthocyanins was the highest in oral digestive fluid，secondary in the intestinal digestive fluid，and least in gastric digestive fluid and intestinal dialysate. Procyanidin was the most abundant in oral digestive fluid，moderate in gastric digestive fluid，and disappeared in the intestinal digestive fluid and intestinal dialysate.Polyphenols had the highest content in intestinal digestive fluids，similar content in oral and gastric digestive fluids，and the lowest content in intestinal dialysate. The simulated digestive fluids scavenged hydroxyl and DPPH radicals in similar trends. The simulated oral digestive fluid had the strongest antioxidant activity，with the IC50 of 71 μL for scavenging hydroxyl radicals，stronger than vitamin C，and the IC50 of 13.7 μL for scavenging DPPH radicals，weaker than vitamin C. The simulated intestinal digestive liquid had the weakest antioxidant ability. However，the digestive fluids showed different ABTS+free radical scavenging activities.Further investigation is needed to study the ability to scavenge superoxide anion radicals.The digestion products of A.melanocarpa extract maintained potential antioxidant activity，which had correlations with the content of active components.