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中文摘要: 该文建立一种间接竞争酶联免疫法（indirect competitive enzyme?linked immunosorbent assay，ic?ELISA）快速测定食品中黄曲霉毒素M1（aflatoxin M1，AFM1）的分析方法。AFM1标准品用甲醇稀释，AFM1标准品与AFM1全抗原竞争结合AFM1单克隆抗体，利用3，3′，5，5′?四甲基联苯胺（3，3′，5，5′?tetramethylbenzidine，TMB）单组分显色液显色后，酶标仪测定吸光度。在优化好的条件下，浓度范围为9.743～2.605×103 pg/mL 时具有良好的线性关系，相关系数（determi?nation coefficient，R2）为0.992 7，检出限IC10为3.84 pg/mL，加标回收率为88.43%～105.75%。该方法适用性好、操作简单、灵敏度高，满足食品中AFM1分析检测的需求。
Abstract:This study established an analytical method for the determination of aflatoxin M1（AFM1）in food by indirect competitive enzyme?linked immunosorbent assay（ic?ELISA）.AFM1 standard，diluted with methanol，competed with AFM1 antigen to bind AFM1 monoclonal antibody.The absorbance was measured by a micro?plate reader after color development with a single?component TMB color?developing solution.Under optimized conditions，AFM1 had a good linear relationship in the concentration range of 9.743-2.605×103 pg/mL，with the determination coefficient（R2）of 0.992 7，the limit of detection IC10 of 3.84 pg/mL，and the recovery rate of 88.43%-105.75%.This determination method had good applicability，simple operation，and high sensitiv?ity，and met the requirements of AFM1 analysis and detection in food.
keywords: indirect competition enzyme⁃linked immunosorbent assay（ic⁃ELISA） aflatoxin M1 rapid detec⁃tion food kits
文章编号：202403025 中图分类号： 文献标志码：
基金项目:国家重点研发计划项目（2017YFC1601101）
| 作者 | 单位 |
| 侯悦1，2，陈瑞鹏2，芦然2，高志贤2，周焕英2*，杨仕平1* | 1.上海师范大学化学与材料科学学院，上海 200000；2.军事科学院军事医学研究院环境医学与作业医学研究所，天津 300050 |
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