Abstract:To investigate the effect of β?1，3/α?1，3?glucan combined with probiotics on the laxative function of mice with constipation mice were randomly divided into five groups：negative control group，model group，1∶1 group of β?1，3/α?1，3?glucan and Lactobacillus parasei，1∶1 group of β?1，3/α?1，3?glucan and Lactobacillus rhamnosus and Lactobacillus rhamnosus，and 1∶1 group of β?1，3/α?1，3?glucan and Bifidobacterium latisticus.Except for the control group，loperamide hydrochloride was used to establish a mouse constipation model in the other groups.The time of first black stool excretion，the number of black stools with in 6 h，the weight and water content of feces，and the ink propulsion rate of the small intestine were observed.The results showed that the three treatment groups could significantly shorten the time of first black stool excretion and increase the weight and number of feces，ink propulsion rate，and water content of feces compared with the model group.Among them，the effect of the 1∶1 group of β?1，3/α?1，3?glucan and Lactobacillus paracasei was better than that of the 1∶1 group of β?1，3/α?1，3?glucan and Lactobacillus rhamnosus，Bifidobacterium lactis.The study showed that β?1，3/α?1，3?glucan combined with probiotics could effectively promote intestinal peristalsis and defecation in mice with constipation and relax the bowel.