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中文摘要: 大豆中富含多种营养物质，其中配基型异黄酮（soybean isoflavone aglycones，SIA）在人体中的吸收利用率最佳，β?葡萄糖苷酶可以将难以利用的糖苷型异黄酮（soybean isoflavone glycoside，SIG）转化为SIA。选取一株前期筛选到的产β?葡萄糖苷酶的植物乳植杆菌c4?3 用于豆浆的发酵，经12 h 发酵后pH 值从6.47 降至4.4；SIA 含量明显升高，其中金雀异黄酮和大豆苷元分别从9.68 mg/L 和13.93 mg/L 增加至26.61 mg/L 和68.43 mg/L。进一步通过非靶向代谢组学研究发现，植物乳植杆菌c4?3发酵豆浆中除异黄酮外，γ?氨基丁酸的含量也上升了44.03%，儿茶素、磷酸吡哆醇的含量分别上升了18.12 倍和1.03 倍。
Abstract:Soybean is rich in isoflavones and other nutrients，of which the soybean isoflavone aglycones（SIA）has the highest absorption and utilization rate in the human body.β?Glucosidases can convert soybean isofla?vone glycoside（SIG）difficult to be assimilated into SIA.A β?glucosidase?producing strain Lactiplantibacillus plantarum c4?3 obtained from previous work was used for the fermentation of soymilk，which decreased the pH from 6.47 to 4.4 after 12 h fermentation.In addition，the fermentation with the strain significantly increased the content of SIA，of which genistein and daidzein were increased from 9.68 mg/L and 13.93 mg/L to 26.61 mg/L and 68.43 mg/L，respectively.The untargeted metabolomics revealed that in addition to isoflavones，the con?tent of γ?aminobutyric acid in the soymilk fermented with L.plantarum c4?3 was increased by 44.03%，and that of catechins and pyridoxine phosphate was increased by 18.12 times and 1.03 times，respectively.
keywords: soybean isoflavones soybean isoflavone glycoside（SIG） soybean isoflavone aglycones（SIA） fermented soymilk untargeted metabolomics
文章编号：202403002 中图分类号： 文献标志码：
基金项目:国家自然科学基金面上项目（32072185）
| 作者 | 单位 |
| 鲍捷1，2，代艺伟1，2，陈映羲1，2，梁会朋1，2，林心萍1，2，张素芳1，2* | 1.大连工业大学国家海洋食品工程技术研究中心，辽宁大连 116034；2.大连工业大学食品学院，辽宁大连 116034 |
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