Abstract:MYB transcription factors are ubiquitous in eukaryotes and play a key role in plant growth，development，and stress responses.Although extensive studies have been carried out for the MYB transcription factors in plants，little is known about the MYB transcription factors in edible mushrooms.A MYB transcription factor gene in Agaricus bisporus that was identified in the previous transcriptome study was cloned and bioinformatically analyzed.The results showed that the gene had a coding region of 1 209 bp，which encoded 402 amino acid residues.The deduced protein had a molecular weight of 45.95 kDa and an isoelectric point of 9.17，being a hydrophilic protein with no transmembrane structure or signal peptide.In addition，the protein had four highly conserved SANT domains，being a 4R-MYB transcription factor.It had the closest relationship with the MYB transcription factor in Leucoagaricus.The secondary structural elements in the deduced protein were mainly random coils and α-helices.The subcellular localization analysis showed that the protein was located in the nucleus.Furthermore，the promoter region of this gene contained multiple cis-acting elements associated with biotic stress responses and hormone responses.