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投稿时间：2022-09-30
投稿时间：2022-09-30
中文摘要: 含有转基因成分的玉米在生产加工过程中核酸成分受到不同程度破坏，增加出口过程中转基因成分检验难度。针对转基因食用玉米淀粉在加工过程中主要环节进行样本收集，使用荧光定量聚合酶链式反应（polymerase chain reaction，PCR）对加工过程中样品内、外源基因不同扩增片段进行定量研究。研究发现，浸泡后的浆料湿磨及精磨分离阶段的样品DNA 降解严重。随着检测转基因成分方法的不断创新和提高及数字PCR 在检测领域的开发和应用，利用数字PCR 方法检验痕量转基因成分，不仅可以定性检测，并且可以对转基因成分含量进行定量检测。
Abstract:The nucleic acid components of corn containing transgenic ingredients are damaged to varying degrees in the process of production and processing，which increases the difficulty of testing transgenic ingredients in the export process. Samples were collected from the main links of transgenic edible corn starch in the processing process，and different amplified fragments of endogenous and exogenous genes in the samples were quantitatively studied by fluorescence quantitative polymerase chain reaction（PCR）. The results showed that DNA degradation was serious in the wet grinding and fine grinding separation stage of the soaked slurry. With the continuous innovation and improvement of methods to detect transgenic components and the development and application of digital PCR in the field of detection，the use of digital PCR methods for the detection of trace transgenic components can not only qualitatively detect but also quantitatively detect the content of transgenic components.
keywords: fluorescence quantitative PCR transgenic edible corn starch DNA degradation production processing
文章编号：202401020 中图分类号： 文献标志码：
基金项目:黑龙江省玉米主食工业化工程技术研究中心开放课题项目（SPKF202021）；海关总署科研项目（2019HK095）
| 作者 | 单位 |
| 甄珍1，窦迎港2，刘晓兰3 * | 1. 哈尔滨海关技术中心齐齐哈尔综合实验室，黑龙江齐齐哈尔 161000；2. 石河子大学化学化工学院/化工绿色过程省部共建国家重点实验室培育基地，新疆石河子 832003；3. 齐齐哈尔大学食品与生物工程学院，黑龙江齐齐哈尔 161000 |
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