Abstract:Color is a factor affecting the quality of of rice bran oil. In this paper，the extraction method of chromogenic substances in rice bran oil was optimized，and the chromogenic substances were separated and identified by high-performance liquid chromatography-tandem mass spectrometry. The strongly polar chromogenic substances in rice bran oil were extracted by liquid-liquid extraction with methanol∶water（8∶2，volume ratio）as extractant. Then，the samples were saponified and the weakly polar chromogenic substances were extracted using n-hexane. Nine categories of substances were identified from the strongly polar and weakly polar extracts separately，37 substances in total. Dihydroisoflavones was the highest in the strongly polar extract，and dihydroflavonols were the main compounds in the weakly polar extract.