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投稿时间：2022-09-02
投稿时间：2022-09-02
中文摘要: 采用响应面法优化循环超声提取枇杷果肉中的三萜酸的工艺条件，在单因素基础上，选择液料比、乙醇浓度、循环转速、超声时间为影响因素，以三萜酸提取量为指标进行响应面分析，并研究三萜酸提取物的抗氧化活性。结果表明，最佳提取工艺参数为液料比15.5∶1（mL/g）、乙醇浓度70.0%、循环转速811 r/min、超声时间43.5 min，在该条件下三萜酸提取量为（1.789±0.080）mg/g；在三萜酸提取物浓度为0.48 mg/mL 时，对ABTS+自由基、DPPH 自由基、·OH、·O2-的清除率分别达到60.11%、77.86%、52.76%、45.34%。对4 种自由基的半抑制浓度（IC50）分别为0.148、0.102、0.382、0.510 mg/mL。
Abstract:Response surface method was employed to optimize the process conditions for extracting triterpenic acids from loquat by circulating ultrasound. Based on the results of single-factor tests，four factors including liquid-solid ratio，ethanol concentration，circulation speed and extraction time were identified as main variables that affected extraction yield，and the amount of extracted triterpenic acids was used as the index for the response surface analysis. Besides，the antioxidant activities of the extracted triterpenic acids were studied. The results showed that the optimal extraction parameters were as follows：liquid-solid ratio of 15.5∶1（mL/g），ethanol concentration of 70.0%，circulation speed of 811 r/min and ultrasonic time of 43.5 min. Under these conditions，the extraction yield of triterpenic acids was（1.789±0.080）mg/g. When the triterpenic acid extract concentration was 0.48 mg/mL，the scavenging rates for ABTS+·，DPPH·，·OH and ·O2- were 60.11%，77.86%，52.76% and 45.34%，respectively. The half maximal inhibitory concentration（IC50）of the four free radicals were 0.148，0.102，0.382 mg/mL and 0.510 mg/mL，respectively.
文章编号：202401014 中图分类号： 文献标志码：
基金项目:四川省自然科学基金项目（2022NSFSC1676）；肉类加工四川省重点实验室开放基金科研项目（22-R-01、22-R-02）；烹饪科学四川省高等学校重点实验室资助项目（PRKX2021Z03）
| 作者 | 单位 |
| 郭明遗1，2，3，李密1，赵志平2，吴华昌1，邓艳1，2 * | 1. 四川旅游学院食品学院，四川成都 610100；2. 成都大学肉类加工四川省重点实验室，四川成都 610106；3. 四川旅游学院烹饪科学四川省高等学校重点实验室，四川成都 610100 |
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