Abstract:In recent years，bioactive peptides have garnered much attention due to their potential health benefits.Enzymatic methods characterized by simple control，high reproducibility，cost-effectiveness，and low energy consumption have been widely employed for the industrial preparation of bioactive peptides.This paper introduced the performance and mechanisms of pretreatment （chemical，enzymatic，or physical）technologies in the enzymatic preparation of active peptides.Additionally，the structural features of the peptides with diverse bioactive functions were expounded from molecular weights and amino acid sequences.Furthermore，the general purification and identification steps for enzymatic products and the methods for evaluating peptide activity were summarized.Finally，the current challenges and future development trends in the enzymatic hydrolysis-based preparation of bioactive peptides were outlined，with a view to providing insights for the development and utilization of bioactive peptides.