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投稿时间：2023-01-13
投稿时间：2023-01-13
中文摘要: 为促进核桃分心木多酚（Diaphragma juglandis Fructus polyphenols，DJF-P）的开发，该文通过对比5 种大孔树脂对DJF-P 静态吸附解吸效果，筛选出AB-8 型大孔树脂最适于纯化DJF-P 溶液，同时优化大孔树脂纯化的单因素试验条件，并通过高效液相色谱法（high performance liquid chromatography，HPLC）进一步对DJF-P 溶液纯化前后的物质组成成分进行分析。结果表明：最佳提取条件为55%乙醇，料液比为1∶15（g/mL），室温下浸提24 h，浓缩，得到DJFP 浓缩液。最佳纯化工艺为55%乙醇稀释DJF-P 浓缩液20 倍，AB-8 型大孔树脂静态吸附解吸时间均为8 h，解吸液为70%乙醇。HPLC 结果表明，DJF-P 含有槲皮素、阿魏酸、芦丁、绿原酸和没食子酸。
Abstract:In order to promote the development of Diaphragma juglandis Fructus polyphenols（DJF-P），This study adopted a comparative analysis of five types of macroporous resins regarding their static adsorption-desorption performance for DJF-P.AB-8 macroporous resin was selected as the most suitable resin for purification of DJF-P solutions，and the single-factor experimental conditions for macroporous resin purification were optimized.The compositional components of DJF-P solutions before and after purification was further analyzed by high performance liquid chromatography（HPLC）.The results indicated that the optimal extraction conditions were 55% ethanol at a solid-liquid ratio of 1∶15（g/mL），extraction at room temperature for 24 h，and subsequent concentration to obtain DJF-P concentrate.The optimal purification process included diluting the concentrated DJF-P solution with 55% ethanol at a 20 fold dilution，with an 8 h of static adsorption-desorption time using AB-8 macroporous resin，and a desorption solution comprising 70% ethanol.HPLC results showed that DJF-P contained quercetin，ferulic acid，rutin，chlorogenic acid，and gallic acid.
文章编号：202324020 中图分类号： 文献标志码：
基金项目:
| 作者 | 单位 |
| 许欢欢1，苗春霖1，宋颖1，何爱民2，3，吉洋洋2，3，牟德华3，高山1* | 1.河北科技大学 食品与生物学院，河北 石家庄 050018；2.河北绿岭康维食品有限公司，河北 邢台 054300；3.河北省（邢台）核桃产业技术研究院，河北 邢台 054300 |
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