Abstract:To understand the microbial pollution and differences in microbial community structures of different types of drinking water，including pipe network tap water and building tank water in urban areas and selfprovided well water in suburban areas，different water samples were analyzed for pH，turbidity，total coliforms，and other conventional water quality indicators，and the microbial community structure was resolved by highthroughput sequencing technology.The results showed that among the three types of drinking water，the total number of colonies detected in the self-provided well water exceeded the national limit of drinking water hygiene standards，and total coliforms and Escherichia coli were detected.In addition，Cu2+in the pipe network tap water samples exceeded the national limit.The microbial community diversity of the self-provided well water samples was the highest，and that of the building tank water was the lowest.The bacterial community structure of different types of drinking water was different.The dominant bacteria for all three types of drinking water included Proteobacteria，Firmicutes，Bacteroidetes，and Actinobacteria.At the genus level，the average relative abundance of Halomonas，Pelagibacterium，and Nesterenkonia was the highest in the building tank water，followed by the pipe network tap water.Escherichia-Shigella had the highest detection rate in the self-provided well water.Through the microbial metabolic function analysis，three types of drinking water samples were found with a small number of metabolic pathways associated with human diseases.Compared with pipe network tap water and building tank water，there were some differences in the water quality and microbial community of self-provided well water，indicating that self-provided well water was more susceptible to external environments and human activities.