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投稿时间：2022-07-06
投稿时间：2022-07-06
中文摘要: 以桦褐孔菌（Inonotus obliquus）菌丝体为试验材料，多糖提取率为评价指标，采用Box-Behnken 响应面法从提取时间、液料比和提取温度3 个因素优化桦褐孔菌菌丝体多糖的提取工艺，并研究粗多糖对α-葡萄糖苷酶和α-淀粉酶活性的影响。结果表明，桦褐孔菌菌丝体多糖的最佳提取条件为提取温度97 ℃、液料比51 ∶1（mL/g）、提取时间1.9 h、提取3 次，在此条件下多糖提取率为（8.02±0.45）%。体外酶抑制试验表明，桦褐孔菌菌丝体多糖浓度为10 mg/mL 时，对α-葡萄糖苷酶和α-淀粉酶的抑制率分别为（77.64±1.78）%和（39.20±1.17）%，二者的半抑制浓度（half maximal inhibitory concentration，IC50）分别为3.07 mg/mL 和17.20 mg/mL，对α-淀粉酶和α-葡萄糖苷酶的抑制作用明显，表明其具有较好的降血糖能力。
Abstract:Polysaccharides were extracted from the mycelia of Inonotus obliquus.With the yield of polysaccharides as the indicator，Box-Behnken design-response surface methodology（RSM）was employed to optimize the extraction conditions including extraction time，liquid-to-solid ratio，and extraction temperature.Furthermore，the effects of the crude polysaccharides on α-glucosidase and α-amylase activities were studied.The results showed that the yield of polysaccharides from the mycelia of I.obliquus reached（8.02±0.45）% when the extraction was carried out at 97 ℃and a liquid-to-solid ratio of 51 ∶1（mL/g）for 1.9 h and three times of extraction.The in vitro enzyme inhibition tests showed that 10 mg/mL crude polysaccharides exerted the inhibition rates of（77.64±1.78）% and（39.20±1.17）%，with the half maximal inhibitory concentrations（IC50）of 3.07 mg/mL and 17.20 mg/mL on α-glucosidase and α-amylase，respectively.The extracted crude polysaccharides had obvious inhibitory effects on α-amylase and α-glucosidase，demonstrating the potential of lowering blood glucose.
keywords: Inonotus obliquus mycelia polysaccharides extraction process α-glucosidase α-amylase blood glucose-lowering
文章编号：202319016 中图分类号： 文献标志码：
基金项目:黑龙江省院所基本应用技术研究专项（ZNJZ2020SW01）；黑龙江省科学院中试熟化项目（ZSSH202005）
| Author Name | Affiliation |
| CHEN Shengyu，ZHANG Shumei，WANG Yuxia，TIAN Yuan，MA Junxiu，HU Jihua，PAN Yu，HAN Zenghua* | Institute of Microbiology，Heilongjiang Academy of Sciences，Harbin 150010，Heilongjiang，China |
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