Abstract:To investigate the immunomodulatory activity of intestinal bacterial in vitro fermentation broths of indigestible glucans（IGs），six IGs（barley β-glucan，laminarin，yeast β-glucan，pachyman，resistant starch，and litesse）were fermented in vitro for 24 h by anaerobic fermentation model using healthy human fecal microbiota，and IG fermentation broths were obtained by sterile filtration.pH and short-chain fatty acid levels were measured.RAW 264.7 macrophages were divided into normal，positive，and IG fermentation broth groups.Cell viability was determined with a CCK-8 kit，and levels of nitric oxide（NO），reactive oxygen species（ROS），and cytokines were analyzed using NO detection kit，flow cytometry，and enzyme-linked immunosorbent assay，respectively.The results suggested that compared with the normal group，all 24 h fermentation broths of six IGs were able to enhance the viability of RAW 264.7 macrophages，production of NO and ROS，and TNF-α release.The intestinal bacterial fermentation broth of each IG had the immunomodulatory ability on RAW 264.7 macrophages.