Abstract:The optimal extraction conditions for Kuding tea（KT）binding polyphenols were selected using response surface methodology based on the results of single-factor experiment.Different types of macroporous resins were used to adsorb KT binding polyphenols to remove salts from crude extracts，and their inhibitory effects on the digestion enzyme α-glucosidase were studied.The results showed that the optimal extraction conditions for KT binding polyphenols were as follows:sodium hydroxide concentration of 8.5 mol/L，liquid-to-material ratio of 43∶1（mL/g），and extraction time of 4.2 h.Under these optimized conditions，the content of polyphenols was determined to be（29.8±2.0）mg/g.Among the seven different types of macroporous resins tested，AB-8 macroporous resin exhibited the highest adsorption rate and desorption rate for KT binding polyphenols.KT binding polyphenols showed significant inhibition on α-glucosidase，with a half-inhibitory concentration of 1.90 mg/mL，reversible inhibition，and non-competitive inhibition with an inhibition constant Ki of 0.60 mg/mL.