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投稿时间：2022-05-03
投稿时间：2022-05-03
中文摘要: 采用单因素试验优化微生物发酵降解不溶性茯苓多糖的最佳发酵条件，在最佳条件下，从发酵液中分离纯化得到水溶性茯苓多糖。采用红外光谱表征其结构，以凝胶渗透色谱法测定其相对分子质量，并对其抗氧化活性进行研究。结果表明：最佳发酵条件为发酵时间36 h、发酵温度26 ℃、初始发酵pH7、转速160 r/min、接种量8%，在该条件下，不溶性茯苓多糖的降解率为60.25%；发酵后水溶性茯苓多糖具有糖类物质的特征结构，相对分子质量为1.51×104 Da，对DPPH 自由基、羟自由基和超氧阴离子自由基清除的半抑制浓度分别为3.79、6.10、3.99 mg/mL。
Abstract:Single factor test was used to optimize the conditions for microbial fermentation of water-insoluble Poria cocos polysaccharides.From the fermentation broth under the optimal conditions，the water-soluble polysaccharides were isolated and purified.The structure and molecular weight of the water-soluble polysaccharides were characterized by infrared spectroscopy and gel permeation chromatography，respectively.Meanwhile，the antioxidant activity of the polysaccharides was examined.The results showed that the optimal conditions were fermentation time 36 h，fermentation temperature 26 ℃，initial pH7，shaker speed 160 r/min，and inoculum volume 8%.Under the conditions，the degradation rate of the water-insoluble polysaccharides was up to 60.25%.The yielded water-soluble polysaccharides had the typical structure of sugars，and the molecular weight was 1.51×104 Da.The soluble polysaccharides scavenged DPPH，hydroxyl radical and superoxide anion with IC50 of 3.79，6.10，3.99 mg/mL，respectively.
keywords: microbial degradation Poria cocos polysaccharides water solubility molecular weight antioxidant
文章编号：202315020 中图分类号： 文献标志码：
基金项目:
| Author Name | Affiliation |
| ZHANG Yixuan，LEI Min，HUANG Jiahao，CAI Jun* | Hubei University of Technology，Wuhan 430068，Hubei，China |
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