Abstract:The mechanism of buckwheat quercetin in the intervention of hepatic lipid metabolism disorders was studied.With the palmitic acid-stimulated mouse primary hepatocytes as a model，transcriptomics was employed to establish a gene pool of primary hepatocytes.The biological role of buckwheat quercetin in alleviating the abnormal lipid metabolism of primary hepatocytes was explored.The results showed that palmitic acid stimulation and quercetin intervention groups differed significantly in gene expression.Quercetin attenuated hypoxiainducible factor-mediated endoplasmic reticulum stress and thus slowed down lipolysis.Furthermore，it reduced the influx of free fatty acids into hepatocytes by blocking their transport，and thus improved insulin sensitivity to inhibit the glucose production pathway in the liver.The findings revealed that buckwheat quercetin ameliorated the abnormal lipid metabolism in primary hepatocytes by slowing down lipolysis.